Description
In this project, we will develop advanced methods for the characterization of extracellular vesicles. We will (i) develop flow cytometric methods for the characterization of vesicles in the size range below 200 nm (binding to magnetic beads, fluorescence triggering and simultaneous detection of surface markers on extracellular vesicles using the MACSPlex technology), (ii) set up a method for the flow cytometric characterization of complement factor and tissue factor-bearing extracellular vesicles (iii) characterize extracellular vesicles after shock wave therapy, (iv) define the impact of extracellular vesicles on the distribution of monocyte subsets and (v) set up methods for the new NTA device.
Details
Duration | 01/01/2023 - 31/12/2024 |
---|---|
Funding | Bundesländer (inkl. deren Stiftungen und Einrichtungen) |
Program | |
Department | |
Principle investigator for the project (University for Continuing Education Krems) | Mag. Rene Weiss, PhD |
Publications
Weiss, R. and Mostageer, M.; Eichhorn, T.; Huber, S.; Egger, D.; Spittler, A.; Tripisciano, C.; Kasper, C.; Weber, V. (2024). The fluorochrome-to-protein ratio is crucial for the flow cytometric detection of tissue factor on extracellular vesicles. Sci Rep, 14: 6419
Lectures
Characterization of tissue factor-bearing extracellular vesicles in COVID-19 provide evidence for platelet-leukocyte aggregate formation
15th ÖGMBT Annual Meeting, 20/09/2023
Characterization of tissue factor-bearing extracellular vesicles in COVID-19 provides evidence for platelet-monocyte aggregate formation
Hyperflow 2023, 12/05/2023