LNP Profiling - Standardized cell-based characterization of lipid nanoparticles for safer, more effective therapies

The aim of this project is to develop an analytical toolkit for evaluating the efficacy, cytotoxicity, off-target effects, immunogenicity, and tissue specificity of lipid nanoparticles (LNPs). By combining cutting-edge molecular biology techniques such as RNA sequencing, quantitative PCR, and fluorescence-based activity assays, with advanced physico-chemical methods, including Taylor dispersion analysis and MALDI imaging, a comprehensive and standardized approach will be established. The use of cell-based assays with primary cells derived from various tissue types enables precise analysis of LNP effects. The goal is to standardize reproducible methods for the assessment of LNP formulations, particularly for RNA-based therapeutics. The interdisciplinary collaboration between RNAnalytics, the University of Applied Sciences Tulln, and the University for Continuing Education Krems aims to integrate expertise in LNP production, cell biology, and molecular analysis. The project will result in the development of a toolkit for evaluating LNP effects, which will subsequently be tested in relevant laboratory environments and made available to both academic and industrial users. The outcomes will make a significant contribution to the development of safe, efficient, and targeted therapeutics, while simultaneously strengthening the innovation potential of Lower Austria.