This project deals with the development and characterization of a cell culture model for Gram-negative sepsis. The model is based on stimulation of monocytes with lipopolysaccharide and the subsequent stimulation of endothelial cells with the cell culture supernatant of the stimulated monocytes. With different read-out assays, such as the secretion of cytokines and the expression of surface adhesion molecules, the activation of the endothelium can be assessed. The model is potentially suited to identify new targets for sepsis therapy as well as to test the effect of modulation of defined mediators on endothelial activation.


Duration 01/01/2007 - 31/01/2010
Funding FFG
Program FFG (Bridge)

Department for Biomedical Research

Center for Biomedical Technology

Principle investigator for the project (University for Continuing Education Krems) Univ.-Prof. Dr. Viktoria Weber
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