Cell-based therapies for regenerating bone

Description

Osteo-integration of bone grafts or bone substitutes is a very long process, which is suboptimal in the majority of clinical situations. We are continuing research into the mechanisms underlying the remodeling at the graft-host interface. Our earlier studies showed that supplementation of blood-derived product either as platelet rich plasma (PRP) or serum albumin can markedly improve bone remodeling. However, in order to create clinically feasible treatment protocols the biology of graft integration must be understood in greater detail and the mode of action of serum-supplemented bone grafts should be clarified. Under the present proposal we aim to investigate the allograft-stem cell composite under in vitro simulated ischemia and test its vascularization potential. Human bone explant will be harvested from femoral heads and exposed to oxygen-glucose deprivation in order to simulate ischemia. Cell viability will be monitored through fluorescent dyes and metabolic assays. Serum albumin and PRP coating will be investigated as potential growth-promoting factors after ischemia. In another line of experiment we shall implant bone chips in chicken egg the chorioallantoic membrane and monitor the vascularization potential. We expect that these experiments will allow a deeper understanding of bone graft remodeling and will lead to the design of better bone substitutes. This project is funded by the NFB (NÖ Forschungs- und Bildungsges.m.b.H.).

Details

Duration 01/12/2013 - 31/05/2017
Funding Bundesländer (inkl. deren Stiftungen und Einrichtungen)
Program Life Science Call NFB
Department

Department for Health Sciences, Medicine and Research

Center for Regenerative Medicine

Principle investigator for the project (Danube University Krems) Univ.-Prof. Dr. Stefan Nehrer
Project members Vivek Jeyakumar, PhD MSc

Publications

Horváthy, D.B.; Schandl, K.; Schwarz, C.M.; Renner, K.; Hornyák, I.; Szabó, B.T.; Niculescu-Morzsa, E.; Nehrer, S.; Dobó-Nagy, C.; Doros, A.; Lacza, Z. (2019). Serum albumin-coated bone allograft (BoneAlbumin) results in faster bone formation and mechanically stronger bone in aging rats. Journal of Tissue Engineering and Regenerative Medicine, Mar;13(3): 10.1002/term.2803

Jeyakumar, V.; Niculescu-Morzsa, E.; Bauer, C.; Lacza, Z.; Nehrer, S. (2019). Redifferentiation of Articular Chondrocytes by Hyperacute Serum and Platelet Rich Plasma in Collagen Type I Hydrogels. International Journal of Molecular Sciences, Jan 14;20(2): 10.3390/ijms20020316

Kjaer, M.; Bachl, N.; Lorenz, C.; Nehrer, S.; Halbwirth, F. (2018). Mechanische Belastung und Bindegewebe. In: Bachl, N.; Löllgen, H.; Tschan, H.; Wackerhage, H.; Wessner, B., Molekulare Sport- und Leistungsphysiologie: 139-164, Springer, Wien

Jeyakumar, V.; Halbwirth, F.; Niculescu-Morzsa, E.; Bauer, C.; Zwickl, H.; Kern, D.; Nehrer, S. (2017). Chondrogenic Gene Expression Differences between Chondrocytes from Osteoarthritic and Non-OA Trauma Joints in a 3D Collagen Type I Hydrogel. Cartilage, 8(2): 191-198

Jeyakumar, V.; Niculescu-Morzsa, E.; Bauer, C.; Lacza, Z.; Nehrer, S. (2017). Platelet-Rich Plasma Supports Proliferation and Redifferentiation of Chondrocytes during In Vitro Expansion. Frontiers in Bioengineering and Biotechnology, 5:75: doi: 10.3389/fbioe.2017.00075

Lectures

Differential Effects of Platelet-Derived Products on ECM turnover by OA Chondrocytes in Collagen Hydrogels

GOTS-Jahreskongress 2017, At Berlin, Germany, 18/06/2017

Differential effects of platelet-derived products on ECM turnover by OA chondrocytes in collagen hydrogels

TERMIS European Chapter Meeting 2017, Davos, Switzerland, 18/06/2017

Effects of Platelet-Derived Products on ECM turnover by OA Chondrocytes in Collagen Hydrogels

ORS 2017, Annual Meeting of the Orthopaedic Research Society, At San Diego, California, United States, 18/03/2017

Chondrogenic differentiation potential of osteoarthritic chondrocytes under human serum from platelet-rich fibrin (SPRF) and platelet-rich plasma (PRP)

TERMIS-EU congress 2016, European Chapter Meeting of the Tissue Engineering and Regenerative Medicine International Society, At Uppsala, Sweden, 18/06/2016

Effects of human serum from platelet-rich fibrin (SPRF) and platelet-rich plasma (PRP) on the chondrogenic differentiation potential of osteoarthritic chondrocytes

Annual Meeting of the Orthopaedic Research Society 2016, 18/03/2016

Chondrocytes from osteoarthritic and healthy joints differ in chondrogenic gene expression on a 3D collagen type I hydrogel

World Conference on Regenerative Medicine, At Leipzig, Germany, 18/10/2015

Donor age variation in human serum derived-platelet rich fibrin influences the proliferation rate of osteoarthritic chondrocytes

Österreichische Gesellschaft für Orthopädie congress 2015, Vienna, Austria, 18/10/2015

Human Platelet-Rich Plasma Enhances Proliferation and Synthesizes sGAG Production of OA Chondrocytes in a Tissue-Engineered Construct

4th TERMIS World Congress 2015, Boston, 08/09/2015

Team

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