The consumption of food containing biogenic amines is reported to be responsible for many pharmacological effects (diarrhea, headache, rhinoconjunctival symptoms, asthma, hypotension, arrhythmia, urticaria, pruritus, flushing) which lead to several types of foodborne disease, including histamine poisoning, mainly from ingestion of certain species of fish (scombroid poisoning), and tyramine toxicity (cheese reaction). Amines have also been implicated as the cause of toxicity to chicks and were also studied as carcinogenic precursors. Nevertheless, the occurrence of symptoms that result from such interactions remains hypothetical, because there are no acceptable clinical studies that demonstrate such reactions. One reason is the lack of reliable test methods. In BIOAMINA we, the Austrian Institute of Technology, Health and Environment Department and the Danube University Krems, Center of Integrated Sensor Systems will contribute to this issue developing a multiplex diagnostic tool that is capable of reliably and simultaneously measuring histamine, tyramine, putrescine and other biogenic amines in test food, serum and urine. For validation of the colorimetric enzyme-based assays and detection systems in clinically relevant samples an oral open food challenge study with healthy volunteers shall be conducted at the Karl Landsteiner Institute St. Poelten. ** This work is partially co-funded by the NÖ Forschungs- und Bildungsges.m.b.H. (NFB) within the Life Science Call.


Duration 01/10/2017 - 31/12/2021
Funding Bundesländer (inkl. deren Stiftungen und Einrichtungen)
Program nfb

Department for Integrated Sensor Systems

Center for Water and Environmental Sensors

Principle investigator for the project (University for Continuing Education Krems) Dipl.-Ing. Dr. Martin Brandl
Project members
Lakshmi Devi Chakkarapani, PhD MSc


Chakkarapani, L.D.; Brandl, M. (2021). Highly sensitive electrochemical detection of tyramine using a poly(toluidine blue)-modified carbon screen-printed electrode. IEEE Sensors Journal, 22: 1005613

Chakkarapani, L.D.; Brandl, M. (2021). Electrochemical Study of Poly(Azure A)-Film Manganese-Hexacyanoferrate-Complex modified Electrodes for Histamine Detection. In: Proceedings in 8th International Electronic Conference on Sensors and Applications: xx, MDPI, online

Chakkarapani, L. D.; Brandl, M. (2020). Carbon Screen-Printed Electrode Coated with Poly (Toluidine blue) as an Electrochemical Sensor for the Detection of Tyramine. MDPI Eng. Proc., 2/51: 10.3390/ecsa-7-08171

Wagner, L.-M.; Pilecky, M.; Mazza, G.; Preininger, C.; Brandl, M. (2018). Simulation and Performance Optimization of an Amperometric Histamine Detection System. MDPI Proceedings, 2018, 2: 853


Fast and Accurate Detection of Hydrogen Peroxide

MNE 2019, 24/09/2019

Simulation and optimization of a screen-printed electrode for chronoamperometric detection of biogenic amines

2nd European Biosensor Symposium 2019, Florenz, Italien, 19/02/2019

Performance Optimization of a Histamine Detection System using electrochemical Simulations

MNE 2018, 26/09/2018

Simulation and Performance Optimization of an Amperometric Histamine Detection System

Eurosensors 2018, 10/09/2018

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