Description
As the incidence of osteoarthritis increased over the last years, development of new therapeutic approaches for osteochondral regeneration come into the focus of tissue engineering research. Besides conventional therapies using patient derived chondrocytes, stem cells became a promising cell source for cartilage repair due to their modulatory properties. But besides benefits of stem cell usage, also drawbacks occurred such as rejection of the cells after transplantation into the defect, teratoma formation or low differentiation capability of stem cells into the desired tissue. To circumvent these limitations, establishment of cell free approaches which comprise desired properties of stem cells are needed. Extracellular vesicles represent a novel mechanism by which signals are communicated among different cell types leading to cellular responses due to their cargo (e.g. proteins, lipids, mRNA,miRNA). It has already been described that extracellular vesicles isolated from body fluids as well as from cell conditioned media are potent mediators of therapeutic signals playing a role in a vast number of diseases including cancer, ischemia, sepsis, rheumatoid arthritis, osteoarthritis among others. The focus of this project lies in evaluating the therapeutic potential of extracellular vesicles isolated from mesenchymal stem cells derived from the Hoffa´s fat pad on processes occurring during osteoarthritis (extracellular matrix degradation, inflammation). The Hoffa´s fat pad is located intra articularly behind and under the patellar. Due to its proximity to the cartilage, mesenchymal stem cells from this adipose tissue are more involved in the development and progression of osteoarthritis as mesenchymal stem cells derived from bone marrow which are often used for cartilage regeneration studies. In addition, the impact of blood derived products on Hoffa´s fat pad mesenchymal stem cells and their extracellular vesicles will be evaluated. Blood derived products such as platelet rich plasma (PRP) are often used in orthopedics with outstanding therapeutic efficacy. Utilization of the two blood derived products PRP and hyperacute serum as a cell culture supplement in order to replace conventionally used animal serum will also aid in translating findings obtained from cell culture experiments from bench to bedside.
Details
| Duration | 01/03/2020 - 28/02/2023 |
|---|---|
| Funding | Bundesländer (inkl. deren Stiftungen und Einrichtungen) |
| Program |
Life Science Call NFB
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| Department | |
| Principle investigator for the project (University for Continuing Education Krems) | Ass.-Prof. Mag. Andrea De Luna, PhD |
Publications
Otahal, A.; Kramer, K.; Neubauer, M.; Gulová, S.; Lacza, Z.; Nehrer, S.; De Luna, A. (2024). Culture of Hoffa fat pad mesenchymal stem/stromal cells on microcarrier suspension in vertical wheel bioreactor for extracellular vesicle production. Stem Cell Research and Therapy, 15: https://doi.org/10.1186/s13287-024-03681-9
Otahal, A.; De Luna, A.; Mobasheri, A.; Nehrer, S. (2023). Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy. In: Humana Press, Cartilage Tissue Engineering: 123-140, Springer Protocols, United States of America
Otahal, A (2023). Extrazelluläre Vesikel, die Botenstoffe der Regeneration. JATROS - Orthopädie & Traumatologie Rheumatologie, 2/2023: 20, Universimed
De Luna, A.; Otahal, A.; Nehrer, S. (2020). Mesenchymal Stromal Cell-Derived Extracellular Vesicles - Silver Linings for Cartilage Regeneration? Frontiers in Cell and Developmental Biology, 8:593386: 10.3389/fcell.2020.593386
Lectures
Extrazelluläre Vesikel - Die Botenstoffe der Regeneration?
Wissenschaftsbotschafter, 31/01/2024
Extracellular vesicles from Hoffa Fat Pad derived MSC - silver linings for cartilage repair?
Jahrestagung des Vienna Center for Engineering in Medicine, 13/12/2023
Hoffa-derived MSC extracellular vesicles in osteoarthritis – friend or foe?
LBG Meeting Innovation in Health Sciences 2023, 02/11/2023
Hoffa-derived MSCs primed with IL1ß in bioreactor culture yield extracellular vesicles hindering chondrocyte recovery
17th World Congress ICRS 2023, 11/09/2023
Hoffa-derived MSCs primed with IL1ß in bioreactor culture yield extracellular vesicles hindering chondrocyte recovery
Small New World 2.0, 04/09/2023
Extracellular vesicles from Hoffa Fat Pad derived mesenchymal stem cells – friends or foe?
Webinar of the Italian Society of Stem Cells, 04/07/2023
Hoffa MSC-Derived Extracellular Vesicles in Osteoarthritis – Friend or Foe?
19th Life Science Meeting 2023, 23/05/2023
Culture of Hoffa-derived MSCs in vertical wheel bioreactor for EV production in comparison to static 2D culture
Annual meeting of the Austrian Cluster for Tissue Regeneration, 03/05/2023
Extracellular vesicles from Hoffa Fat Pad MSCs favor the osteoarthritic phenotype of chondrocytes
Annual Meeting of the Austrian Cluster of Tissue Regeneration, 03/05/2023
Extracellular vesicles in blood derived products
ICRS World Congress, 12/04/2023
Isolation and culture of Hoffa-derived adipose MSCs for extracellular vesicle production in a vertical wheel bioreactorÖGMBT W
ÖGMBT Workshop „Advanced Cell Culture Technologies", 17/03/2023
Polybrene enhances uptake of extracellular vesicles derived from Hoffa mesenchymal stromal cells into primary osteoarthritic chondrocytes
Small New World Joint Meeting of ASEV & GSEV, 29/10/2022
Extracellular vesicles from blood derived products for cartilage repair
ASEV/GSEV Joint Meeting, 28/10/2022
Functional repertoire of EV-associated miRNA profiles from blood-derived products
48th Annual ESAO Congress, 10/09/2022
Extracellular Vesicles in Blood-Derived Products for Cartilage Repair? - a status report
Annual Meeting of the Austrian Cluster of Tissue Regeneration, 27/04/2022
Association of regenerative capacity of blood EVs on primary osteoarthritic chondrocytes with EV associated miRNAs
ICRS 2022 World Congress Berlin, 14/04/2022
Extracellular Vesicles in Blood-Derived Products for Cartilage Repair
ICRS World Congress, 14/04/2022
Extracellular vesicle-associated miRNA cargo could explain regenerative capacities of blood-derived extracellular vesicles on primary osteoarthritic chondrocytes
OARSI 2022 World Congress, 08/04/2022
Evidenz zum Einsatz der regenerativen Medizin?
38. AGA Kongress, 10/09/2021