Research

Center for Biomedical Technology

The focus of our research is the development of extracorporeal blood purification techniques, in particular adsorption technologies for liver support and the supportive therapy of sepsis.

In this context, we are working in the field of biocompatibility of biomaterials, on the influence of anticoagulation procedures on the blood compatibility of extracorpoeral procedures, furthermore with cell culture models to study the activation of the endothelium under septic conditions, as well as with the role and biological activity of extracellular vesicles.

The aim of the Christian Doppler Laboratory for Innovative Therapy Approaches in Sepsis is to develop supportive extracorpoeral sepsis therapies. The CD Laboratory focuses on the heterogeneity of septic patients and the role of extracellular vesicles in immunomodulation.

Excellent infrastructure and a constantly evolving range of methods in the field of medical process engineering, cell culture and medical biochemistry as well as cooperation with clinical partners enable the transfer of research work from the basis into the application.

Infrastructure

Our center has a laboratory area of ​​approx. 500 m² with equipment in the fields of biochemistry, medical process engineering, microbiology and cell culture. The laboratories are equipped for work on biological safety levels 1 and 2. A Core Facility with advanced imaging equipment is available at Campus Krems.

  • Autoanalyzer for clinical parameters (cobas/Roche C311)
  • Blood count (Sysmex KX-21N, Sysmex XS-800i)
  • Chromatographic separation methods (Colyumn Crhomatography, HPLC)
  • Flow Cytometer (Cytoflex LX)
  • Elektron microscope with EDX (Hitachi FlexSEM 1000) and Sputter-Coater
  • Gel documentation system (BioRad ChemiDocTM XRS)
  • Coagulation analyzer (Sysmex CA-560)
  • Immunofluorescence microscopy (Olympus BX51)
  • Luminex Bead Array
  • PCR (Roche Light Cycler 96)
  • Cell culture models (ibidi µ-slide microfluidic system)
  • Confocal Microscopy (Leica)
  • Nanoparticle Tracking Analysis (Particle Metrix)


We have access to the following methods via our cooperation partners:

  • Atomic Force Micrsocopy
  • Environmental Scanning Electron Microscope (ESEM)
  • Cryo-Electron microscopy (Tecnai F30 Polara transmission electron microscope)
  • Imaging Flow Cytometry (ImageStreamx MkII cytometer, Amnis)

Methods

  • Size exclusion chromatography
  • Quantification of cytokines und mediators: ELISA, Luminex Bead Array
  • Blood count, coagulation analysis, thrombin generation
  • Characterization of adsorbents for extracorporeal blood purification:
    • Adsorption capazity
    • Adsorptions kinetics
    • Pore structure
    • Surface characterization
    • Size exclusion
    • Size distribution
  • Charakterisation of extracellular vesicles
    • Nanoparticle Tracking Analysis
    • Flow cytometry
    • Imaging Flow Cytometry
    • Blood material interactions
    • Activation of the coagulation system
    • Immunomodulation
  • Charakterisation of membranes:
    • Sieving coefficient
    • Transmembrane pressure
    • (Ultra)Filtrations rates
  • Impact of the anticoagulation on blood compatibility
  • Electrophoresis & Western Blotting
  • Endotoxin quantification with the Limulus-Amebocyte-Lysate Test
  • Characterization of polymers regarding blood cell activation
  • Cell culture models for the characterization of the endothelial activation under septic conditions
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